The Importance of
Mutational Testing

Molecular testing can provide comprehensive and meaningful prognostic information. In this era of personalized patient care, advances in mutational testing have improved access to molecular data for diagnosis, risk stratification and clinical decision-making.1

Several gene mutations have been identified among patients with MDS that may, in part, contribute to the clinical heterogeneity of the disease course, and thereby influence the prognosis of patients.2

The NCCN Clinical Practice Guidelines in Oncology (NCCN guidelines®) for Acute Myeloid Leukemia (AML) have also evolved over time to incorporate molecular mutations into risk stratification (along with morphology and cytogenetics) and to emphasize the need to expedite mutational testing results for mutations including IDH1 and IDH2.1

Many testing laboratories have improved turnaround times in some cases to as few as 3-5 days, but real-world data suggests that a delay of a week or two had no impact on patient outcomes.3–7,9,10

A Short Testing Delay Has No
Impact on Outcomes

A Short Testing Delay Has No Impact on Outcomes

A large trial utilizing real-world data explored the impact of a short delay for testing on patient outcomes in cases of AML that do not require immediate action, i.e., leukostasis.5 The results revealed that a short delay for mutational testing to personalize AML management had no adverse effect on patient survival or remission rates.5 Patients were categorized based on the time from diagnosis to treatment start (TDT) – 0-5 days, 6-10 days, 11-15 days, or >15 days.5 The 2-year overall survival (unadjusted) for each TDT group was 51%, 48%, 44%, and 50%, respectively, indicating no significant differences between the groups.5 Furthermore, there were no significant differences in overall survival, even when stratified for age (up to 60 years vs. over 60 years) or white blood cell count (low vs. high).5 This suggests that taking a slight pause to determine the best course of action in AML management may be a viable option without negatively impacting patient outcomes.5

“Kaplan-Meier estimates of OS according to TDT groups. (A) All patients. (B) In patients ≤60 years. (C) In patients >60 years.”5

The Beat AML Master trial further validated the idea that taking time for mutational testing can optimize the management of AML.6 In this trial, mutational testing was conducted within seven days to customize care based on individual mutational results.6 The study found that a seven-day delay to perform detailed molecular testing did not negatively impact mortality and overall survival rates.6

These findings reiterate that a short delay for mutational testing can be safely implemented to determine a personalized management approach for AML patients.6

Taking a slight pause to determine the best course of
action in AML management may be a viable option
without negatively impacting patient outcomes.5,6

NCCN Guidelines®
Recommendations in AML

NCCN Guidelines® Recommendations in AML

The recent NCCN Guidelines in AML underscore the critical importance of expediting mutational testing results, especially in cases involving IDH1 or IDH2 mutations.1

Molecular Profiling Is Part of the Standard Workup as Several Gene Mutations Can Affect Prognosis

“Identification of mutations that carry prognostic and therapeutic impact is rendering molecular profiling for all AML cases a standard part of the diagnostic workup. In addition to basic cytogenetic analysis, new molecular markers can help refine prognostics groups, particularly in patients with a normal karyotype.”1

“Multi-gene molecular profiling/targeted NGS (including IDH1/IDH2, FLT3 mutations) is suggested…Molecular testing should be repeated at each relapse or progression.”1

Expedite Testing for Immediately Actionable Mutations While Adding Disease Control

“…Test results of molecular and cytogenetic analyses of immediately actionable genes or chromosomal abnormalities (eg, CBF, FLT3 [ITD or TKD], NPM1, IDH1, or IDH2) should be expedited.”1

“For patients with hyperleukocytosis…or leukapheresis, [disease control] may be considered prior to receiving results.”1

Expanded Availability of Commercial Mutational Testing Labs

“Tests for these molecular markers are now available in commercial reference laboratories and in referral centers. Therefore, it is important for physicians to confer with the local pathologist on how to optimize sample collection from the time of diagnosis for subsequent molecular diagnostic tests.”1

NCCN Guidelines®
Recommendations in MDS

NCCN Guidelines® Recommendations in MDS

Mutational testing is highly recommended in MDS, and includes testing for IDH1 and IDH2 mutations.2

“Genetic testing for somatic mutations (i.e., acquired mutations) in genes associated with myelodysplastic syndromes (MDS) is highly recommended.”2a

“It is recommended to repeat molecular testing to identify targetable mutations.”2b

Despite recommendations, not all AML and MDS patients receive the needed tests, especially in the community setting.8,9

Given the diversity of mutations impacting MDS and AML, accurate assessment of each patient’s genotype is crucial for making informed clinical decisions.1 While full NGS sequencing can be used for this analysis, rapid tests may also be available in as few as 3-5 days, offering valuable information for personalized patient care.3,4,7,9,10

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a at diagnosis
b at relapse after allo-HCT in patients with higher-risk MDS

References:

1. Referenced with permission from the NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines®) for Acute Myeloid Leukemia V.6.2023. © National Comprehensive Cancer Network, Inc. 2023. All rights reserved. Accessed October 25, 2023. To view the most recent and complete version of the guideline, go online to NCCN.org. 2. Referenced with permission from the NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines®) for Myelodysplastic Syndromes V.3.2023. © National Comprehensive Cancer Network, Inc. 2023. All rights reserved. Accessed November 10, 2023. To view the most recent and complete version of the guideline, go online to NCCN.org. 3. Duncavage EJ, et al. Blood. 2022;140(21):2228-2247. doi: 10.1182/blood.2022015853 4. Megías-Vericat JE, Ballesta-López O, Barragán E, Montesinos P. BLCTT. 2019; 9:19-32. doi:10.2147/BLCTT.S177913 5. Röllig C, Kramer M, Schliemann C, et al. Blood. 2020;136(7):823-830. doi:10.1182/blood.2019004583 6. Burd A, Levine RL, Ruppert AS, et al. Nat Med. 2020;26(12):1852-1858. doi:10.1038/s41591-020-1089-8 7. Nelson EJ, et al. Mol Diagn Ther. 2023;27:371-381. doi: 10.1007/s40291-022-00638-7. 8. Lin TL, Williams T, He J, et al. Cancer Med. 2015;4(4):519-522. doi:10.1002/cam4.406 9. Pollyea DA, George TI, Abedi M, et al. eJHaem. 2020;1(1):58-68. doi:10.1002/jha2.16 10. Guijarro F, et al. Curr. Oncol. 2023;30:5201-5213. doi: 10.3390/curroncol30060395

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